JUNE 2021
B. Sc. (VI Semester) Examination
BOTANY
Paper: BOT-363
(Molecular Biology, Plant Biotechnology and Bioinformatics)
Full Marks : 80
Pass Marks : 35%
Time : Three Hours
Notes: (i) Answer all questions.
(ii) The figures in the margin indicate full marks for the questions
1. Give a brief account of any five of the following: 2x5= 10
(a) Central dogma
(b) Spontaneous mutation
(c) Wobble hypothesis
(d) t-RNA
(e) RNA polymerase
(f) Pyrimidines
(g) Somaclonal variation
2. Answer any six of the following:5x6= 30
(a) Discuss in detail the different forms of DNA.
(b) Briefly discuss the various applications of Bioinformatics.
(c) What is a basal medium?
(d) Discuss briefly the mechanism of DNA replication.
(e) What are the objectives of tissue culture?
(f) Describe significance of transgenic plants.
(g) Explain point mutation and frame shift mutation.
3. Answer any four of the following:10x4 = 40
(a) Briefly describe prokaryotic protein synthesis
(b) What is a cloning vector? Describe a cloning vector studies by you.
(c) How synthetic seeds are produced?
(d) Write a note on micro-propagation
(e) What is protoplast fusion? Describe the process of protoplast fusion in laboratory.
(f) Write the various methods of laboratory sterilization technique in detail.
