JUNE 2021
B. Sc. (VI Semester) Examination
BOTANY
Paper: BOT-362
(Biochemistry and Biological Techniques)
Full Marks : 80
Pass Marks : 35%
Time : Three Hours
Notes: (i) Answer all questions as stated.
(ii) The figures in the margin indicate full marks for the questions
1. Answer any five of the following: 2x5= 10
(a) What is DNA finger printing?
(b) Chemical structure of Amylose
(c) Co-factors
(d) Resolving power
(e) Denitrification
(f) Nucleotide and Nucleoside
2. Answer any six of the following: 5x6= 30
(a) Working principle of TLC
(b) Secondary structure of ZRNA. Draw and explain.
(c) Nomenclature and classification of Enzymes
(d) B-oxidation pathway
(e) Electron Microscopy
(f) Differentiate monosaccharide and disaccharide with examples.
(g) Ammonification and Nitrification
(h) Applications of Autoradiography
3. Answer any four of the following: 10x4= 40
(a) Define DNA sequencing. Discuss the Sanger method of DNA sequencing.
(b) Discuss the mechanisms of Enzyme action.
(c) Describe the physical and chemical methods of sterilization.
(d) Differentiate between reducing and non-reducing sugars with examples.
(e) Discuss polymerase chain reaction. Write about its merits.
(f) Differentiate and discuss the 2 degree and 3 degree structure of amino acids.
