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B.Sc (VI Semester) Examination
BOTANY
Paper : BOT–363
( Molecular Biology, Plant Biotechnology and Bioinformatics )
Full Marks : 80
Pass Marks : 35%
Time : Three Hours
Note : 1. Answer all questions.
2. The figures in the margin indicate full marks for the questions.
1. Give brief accounts of the following (any five) : 2×5=10
(a) Central dogma
(b) Cellular totipotency
(c) Ex-plants
(d) Purines
(e) Spontaneous mutations
(f) Particle gun method
(g) DNA helicases
(h) Rho factor
(i) Transgenic plant
2. Answer any six from the following : 5×6=30
(a) Discuss the importance of plant tissue culture.
(b) What are the differences between Z-DNA and B-DNA?
(c) Distinguish between missense and non-sense mutation.
(d) Discuss the process of transcription.
(e) Differentiate between repressors and inducers.
(f) Discuss the main features of genetic code.
(g) Describe the structure and functions of t-RNA.
(h) What is a synthetic medium?
(i) Differentiate between prokaryotic and eukaryotic genome.
3. Answer any four from the following : 10×4=40
(a) What is a cloning vector? Describe in detail a cloning vector studied by you.
(b) Write the various methods of laboratory sterilization technique.
(c) What is micropropagation? Describe briefly the techniques of micropropagation.
(d) Describe the operon model for regulation of gene activity.
(e) Briefly explain the various applications of bioinformatics.
(f) What are physical mutagens? Discuss the role of radiations as mutagens.
(g) Describe the replication of DNA.
