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BSc (VI Semester) Examination
BOTANY
Paper : BOT–362
( Biochemistry and Biological Techniques )
Full Marks : 80
Pass Marks : 35%
Time : Three Hours
Note : 1. Answer all questions.
2. The figures in the margin indicate full marks for the questions.
1. Write short notes on any five of the following : 2×5=10
(a) Covalent bond and its energy
(b) Disaccharide sugars
(c) Saturated fatty acids
(d) pH meter
(e) Cofactor
(f) Cell-fixation
(g) Paper chromatography
(h) Thin-layer chromatography
2. Write on any six of the following : 5×6=30
(a) Reducing and non-reducing sugars
(b) Phase-contrast microscope
(c) Properties of water as biological solvent
(d) Demerits of paper chromatography
(e) Physical and chemical properties of nucleic acid
(f) Prosthetic group
(g) TEM
(h) Sterilization techniques
(i) Physical properties of nucleic acid
3. Answer any four of the following : 10×4=40
(a) Give the general account of nitrate metabolism.
(b) What is spectrophotometer? Describe the working principle of spectrophotometer and its applications.
(c) Define electrophoresis. Describe the SDS-PAGE gel electrophoresis and write its merits.
(d) What is chromatography? Give the working principle and application of column chromatography.
(e) Write short notes on the following :
(i) Principle of centrifuge
(ii) Fluorescence microscope
(f) What is enzyme? Mention the types and properties of enzymes.
(g) Explain the methods for permanent slide preparation.
